Next-generation sequencing (NGS) has transformed the landscape of genomics, enabling researchers to sequence DNA rapidly and cost-effectively. However, sequencing entire genomes can be inefficient and expensive for many applications. Target enrichment methods allow researchers to focus on specific regions of interest, enhancing the efficiency and depth of sequencing.
Part 4: Troubleshooting a failed or poor quality NGS run can be a headache if you are unable to identify the cause. Important quality metrics are generated by the NGS instrument which can indicate issues with the sequencing chemistry or with a particular sample library. This post will help you know what to look out for when interpreting these QC metrics.
Part 3: NGS Target Enrichment quality metrices. If you are performing NGS target enrichment assays in your lab, make sure to understand a few key quality metrices.
SPRI beads can be used in a variety of NGS library prep chemistries and are compatible with both manual and automated processing. Their non-specific and reversible binding properties give scientists a tool for predictable and consistent size selection with high recovery of amplicons > 100 bp.
Agilent Technologies Introduces New High-Sensitivity NGS Target Enrichment Solution New Solution Streamlines Workflow and Eliminates Sequencing Errors SANTA CLARA, Calif., July 17, 2017 Agilent Technologies Inc. today introduced its newest next-generation sequencing (NGS) library prep solution — Agilent SureSelectXT HS — a complete target enrichment research solution that provides total workflow management for laboratories, from QC…