A multiplex dPCR solution to modern day molecular biology riddles.
Polymerase Chain Reaction (PCR) is a technology that has revolutionised molecular biology for the better, enabling scientists to detect, amplify and quantify low concentrations of DNA and RNA for a variety of applications, ranging from gene expression to genotyping and more. As with all technologies, there is continuous development over time. For PCR technology, digital PCR (dPCR) was the next step in the evolution. This technology was designed to enable the absolute quantification of nucleic acids from difficult sample types (environmental and blood) at ultra-low concentrations (cfDNA), enabling applications that were not possible before.
Despite technology developments, workflows remained expensive, labor intensive and error prone leading to lower quality data generation. Furthermore multiplexing remained limited due to cost, system and chemistry restrictions. Stilla Technologies, the Multiplex dPCR company, have made some tremendous developments in addressing these challenges for the scientific community. We are proud to introduce the new Stilla Nio range of crystal digital PCR systems, for fully automated dPCR applications of varying throughput.
Which Nio is the right fit for you?
All-in-One dPCR system
Remote access
Simplest dPCR workflow, 5 min hands-on time / 2h30 min time-to-results
The Nio systems from Stilla Technologies offer an impressive 7-color detection capability, enabling multiplexing of up to 21 targets using advanced color combination strategies. Assay transfer between systems is seamless, minimising hands-on time with automated plate processing and continuous loading functionality—all within a compact, bench-top digital PCR (dPCR) system. Coupled with Stilla’s novel Flex Probe technology, these features not only increase system throughput but also reduce the cost per reaction.
The Nio E model processes up to 48 samples per run (3 chips of 16 samples each), while the Nio and Nio+ models accommodate 192 samples (12 chips of 16 samples each) and 384 samples (24 chips of 16 samples each), respectively. Additionally, each chip can run its own unique PCR program, enhancing both system and assay flexibility to meet diverse user needs.
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