Part 4: Troubleshooting a failed or poor quality NGS run can be a headache if you are unable to identify the cause. Important quality metrics are generated by the NGS instrument which can indicate issues with the sequencing chemistry or with a particular sample library. This post will help you know what to look out for when interpreting these QC metrics.
Part 3: NGS Target Enrichment quality metrices. If you are performing NGS target enrichment assays in your lab, make sure to understand a few key quality metrices.
SPRI beads can be used in a variety of NGS library prep chemistries and are compatible with both manual and automated processing. Their non-specific and reversible binding properties give scientists a tool for predictable and consistent size selection with high recovery of amplicons > 100 bp.
Performing NGS experiments? This guide will help make sure you have the required reagents and equipment to get started as well as breakdown a few of the lesser-known topics in NGS.