Part 3: NGS Target Enrichment quality metrices. If you are performing NGS target enrichment assays in your lab, make sure to understand a few key quality metrices.
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In light of the urgent need of novel diagnostic assays to come to market for COVID-19 testing, it is important not to forget about assay quality and accuracy, and this applies to ALL diagnostic assays. Assays need to be properly validated in order to ensure that accurate and appropriate results are produced and reported for each patient.
SPRI beads can be used in a variety of NGS library prep chemistries and are compatible with both manual and automated processing. Their non-specific and reversible binding properties give scientists a tool for predictable and consistent size selection with high recovery of amplicons > 100 bp.