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Agilent Community Design Infectious Disease Pathogen NGS Panels

Exciting news from Agilent! Pathogen enrichment straight from clinical samples is now possible!

 

The ability to perform whole genome sequencing of viruses and bacteria directly from clinical samples is important for understanding the genetics of host-pathogen interactions. Due to the much smaller size of pathogen genomes, the proportion of host nucleic acid in an extract massively outweighs that of pathogen nucleic acid.

To overcome this, the research group led by Professor Judith Breuer at University College London has pioneered the enrichment and sequencing of whole pathogen genomes directly from clinical research material using custom SureSelect NGS panels.

 

“SureSelect target enrichment enables highly efficient whole pathogen genome sequencing without the need for prior culture or PCR. The robust and consistent performance of SureSelect is why we’ve been depending on it for over 10 years.“

 

The custom designed SureSelect NGS panels targeting the genomes of pathogenic bacteria and viruses provide an effective way to capture nucleic acid material directly from various clinical research samples including blood, sera, plasma, stool, and nasopharyngeal aspirates. This obviates the need of prior enrichment of pathogen by culture or PCR. The SureSelect NGS panel designs created by Professor Breuer’s group are listed in the table below and are now available from Diagnostech. The panels are research tools to study pathogen genome sequences in the context of disease, antimicrobial resistance, treatment and vaccination.

 

Visit the Agilent Infectious Disease Design Page, or email us at info@diagnostech.co.za for more information! 

 

Table 1. The Agilent SureSelect Community Infectious Disease Pathogen NGS panels 

Part Number Design Name Targeted Pathogen
RNA Viruses
5191-6721 SureSelect XT Community Design FluA and FluB Influenza Virus A and B
5191-6726 SureSelect XT Community Design PanEnteroRhino Enterovirus A – D and Rhinovirus
5191-6716 SureSelect XT Community Design PanEnterovirus Enterovirus
5191-6719 SureSelect XT Community Design PanHCV Hepatitis C Virus (HCV)
5191-6709 SureSelect XT Community Design PanHIV1 Human Immunodeficiency Virus 1 (HIV-1)
5191-6706 SureSelect XT Community Design PanHIV2 Human Immunodeficiency Virus 2 (HIV-2)
5191-6714 SureSelect XT Community Design PanNoro Norovirus
5191-6727 SureSelect XT Community Design PanParaflu ParaInfluenza 1 – 3
5191-6722 SureSelect XT Community Design PanRespiratoryViruses Parainfluenza 1 – 3, Respiratory Syncytial Virus A and B, and Metapneumovirus
5191-6725 SureSelect XT Community Design PanRSV Respiratory Syncytial Virus A and B (RSV-A and RSV-B)
DNA Viruses
5191-6707 SureSelect XT Community Design CMV Human Cytomegalovirus (HCMV)
5191-6711 SureSelect XT Community Design PanHHV6 Human Herpes Virus 6A and B (HHV-6A and 6B)
5191-6713 SureSelect XT Community Design PanAdenovirus Adenovirus
5191-6717 SureSelect XT Community Design PanEBV Epstein-Barr Virus (EBV)
5191-6720 SureSelect XT Community Design PanHBV Hepatitis B Virus (HBV)
5191-6728 SureSelect XT Community Design PanHumanPolyomaviruses Human Polyomaviruses
5191-6715 SureSelect XT Community Design PanHSV1and2 Herpes Simplex Virus 1 and 2 (HSV-1 and HSV-2)
5191-6718 SureSelect XT Community Design VZV Varicella Zoster Virus (VZV)
Bacteria
5191-6708 SureSelect XT Community Design Ctrachomatis Chlamydia trachomatis
5191-6724 SureSelect XT Community Design Lpneumophila Legionella pneumophila
5191-6710 SureSelect XT Community Design Mtuberculosis Mycobacterium tuberculosis
5191-6723 SureSelect XT Community Design Mtuberculosis Light Mycobacterium tuberculosis, selected targets
5191-6712 SureSelect XT Community Design Nmeningitidis  Neisseria meningitidis